Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Validation of the optimal working concentration and treatment of UDCA to inhibit FIPV proliferation. ( A ) CCK-8 assay for the effect of ursodeoxycholic acid on the activity of CRFK-PHBLV-N cells. ( B ) FIPV N gene level after the action of ursodeoxycholic acid. ( C ) Western blotting to detect the changes of FIPV N protein. ( D ) Gray scale of the FIPV N protein change analysis. ( E ) CRFK-PHBLV-N cells in FIPV IFA results graph. ( F ) CRFK-PHBLV-N cells in FIPV fluorescence intensity analysis. **** P < 0.0001.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Biomarker Discovery, Concentration Assay, CCK-8 Assay, Activity Assay, Western Blot, Fluorescence

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Changes in FIPV N gene copy number and protein expression at different stages after ursodeoxycholic acid treatment. ( A ) Graph of different stages of treatment. ( B ) Graph of FIPV N gene copy number results in CRFK-PHBLV-N cells at different stages. ( C ) Graph of FIPV results in CRFK-PHBLV-N cells at different stages. ( D ) Grayscale analysis of FIPV in CRFK-PHBLV-N cells at different stages. ( E ) Negative staining electron micrograph of FIPV virus particles. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; and ns, not significant.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Expressing, Negative Staining, Virus

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Screening of signaling pathways associated with anti-FIPV infection after UDCA co-treatment based on transcriptomic data. ( A ) KEGG pathway enrichment analysis. ( B ) Differential gene volcano plot. ( C ) Correlation analysis of FIPV-UDCA 200 μg/mol co-treatment with antiviral gene expression. ( D ) IFN-β ELISA results in CRFK-PHBLV-N cells. * P < 0.05; ** P < 0.01; *** P < 0.001; and **** P < 0.0001.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Protein-Protein interactions, Infection, Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Changes in STAT1 phosphorylation and nuclear translocation levels in CRFK-PHBLV-N cells after ursodeoxycholic acid treatment. ( A ) STAT1 Western blot results in CRFK-PHBLV-N cells. ( B ) Grayscale analysis of p-STAT1/STAT1 protein in CRFK-PHBLV-N cells. ( C ) CRFK-STAT1 IFA results in PHBLV-N cells. ( D ) STAT1 fluorescence intensity analysis in CRFK-PHBLV-N cells. ** P < 0.01; *** P < 0.001; and **** P < 0.0001.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Fluorescence

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Changes in TLR3 protein, IRF3 phosphorylation, and nuclear translocation levels in CRFK-PHBLV-N cells after ursodeoxycholic acid treatment. ( A ) Graph of TLR3 and IRF3 Western blot results in CRFK-PHBLV-N cells. ( B ) Graph of TLR3 protein grayscale analysis in CRFK-PHBLV-N cells. ( C ) Grayscale analysis plot of p-IRF3/IRF3 protein in CRFK-PHBLV-N cells. ( D ) Graph of IRF3 IFA results in CRFK-PHBLV-N cells. ( E ) Graph of IRF3 fluorescence intensity analysis in CRFK-PHBLV-N cells. ** P < 0.01; *** P < 0.001; and **** P < 0.0001.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Fluorescence

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Changes in MX1 and ISG15 protein levels in CRFK-PHBLV-N cells after ursodeoxycholic acid treatment. ( A ) Western blot results of MX1 and ISG15 in CRFK-PHBLV-N cells. ( B ) Grayscale analysis of MX1 protein in CRFK-PHBLV-N cells. ( C ) Grayscale analysis of ISG15 protein in CRFK-PHBLV-N cells. ISG15 protein grayscale analysis graph in CRFK-PHBLV-N cells. * P < 0.05; ** P < 0.01; *** P < 0.001; and **** P < 0.0001.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: Western Blot

Journal: Microbiology Spectrum

Article Title: Ursodeoxycholic acid inhibits feline infectious peritonitis virus infection through activating JAK-STAT signaling pathway-induced type I interferon

doi: 10.1128/spectrum.03633-25

Figure Lengend Snippet: Diagram of UDCA anti-FIPV mechanism through JAK-STAT signaling pathway.

Article Snippet: Ursodeoxycholic acid was selected for this study and was provided by MedChemExpress, a life science reagent service provider.

Techniques: